Effect of N-P ration on biomass productivity and lipid content on Skeletonema costatum
The concept of constant element composition of planktonic biomass in the oceans of C:N:P of 106:16:1 (by atoms) was developed by Redfield (1935) and has been a cornerstone in algal physiology and oceanography. Since the nitrate-to-phosphate ratio of the deep ocean closely resembles particulate N:P ratio, it has been inferred that both elements may potentially limit phytoplankton growth.
In this research we will examine how variations in the nitrate-to-phosphate ratio of the growth medium affect the particulate N:P in batch cultures of Skeletonema costatum at irradiances that limit and saturate growth rate. Batch cultures have the advantage of mimicking the onset of nutrient limitation, while the cells are in a transient state. The main focus is the extent to which the elemental stoichiometry of phytoplankton, at various growth stages reflects the nutrient elemental composition of the culture medium. In tandem with the increase of biomass, the decline of dissolved inorganic N and P will be monitored. Finally the changes in composition of Skeletonema costatum during the course of the batch culture will be tested.
Research type: literature study, experiments (HZ, Vlissingen)
Research level: BSc./ MSc. internship / final thesis
Prerequisite: good understanding of algae cultivation; biochemistry; good analytical skills; communicative; independent.
Partners: Wageningen UR, several Small and Medium (Aquaculture) Enterprises, national and international knowledge partners, Centre of Expertise Delta Technology
Researcher involved: research group aquaculture (Christos Latsos)
Period: 2nd semester 2017-2018
Status: Open